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Thứ Hai, 24 Tháng Bảy 2017 / Xuất bản trong chuyên mục Giới thiệu chuyên gia

Nghiên cứu chế tạo hạt nano mang kháng nguyên có khả năng gây đáp ứng miễn dịch

Chủ nhiệm đề tài: PGS.TS. NCVC. Lê Quang Huấn

Đơn vị thực hiện: Viện Công nghệ Sinh học

Thời gian thực hiện: 2013-2014

Hình ảnh nổi bật

Mục tiêu đề tài: Chế tạo được hạt nano mang kháng nguyên và phân tử kích hoạt miễn dịch khác nhau có khả năng gây đáp ứng miễn dịch ở động vật thực nghiệm

Kết quả đạt được

Về khoa học: Đề tài đã xây dựng được quy trình tạo hạt nano chitosan mang gen mã hóa kháng nguyên ung thư và phân tử với cấu trúc CpG có khả năng tăng cường đáp ứng miễn dịch khi thử nghiệm trên thỏ thí nghiệm. Kháng thể thu nhận ở thỏ khi gây miễn dịch bằng nguyên tái tổ hợp polyepitope EPCA-2 có khả năng phát hiện kháng nguyên EPCA-2 tương đương với KIT ELISA thương mại CUSABIO, CSB – EQ 027679HU.

Về ứng dụng: Kháng thể kháng EPCA-2 thu nhận được sau khi gây miễn dịch ở thỏ bằng kháng nguyên tái tổ hợp chứa các epitope EPCA-2.22 và EPCA-2.19 có khả năng ứng dụng để xác định hàm lượng kháng nguyên EPCA-2 trong huyết thanh bệnh nhân để chẩn đoán và theo dõi điều trị đối với ung thư tiền liệt tuyến (đã thử nghiệm đối với 110 mẫu huyết thanh của người bệnh và người khỏe mạnh).

Đóng góp mới :(1) Đề tài đã tạo được hạt nano chitosan mang các gen mã hóa kháng nguyên và các cấu trúc CpG tăng cường đáp ứng miễn dịch; (2) Đề tài đã chứng minh khả năng thu nhận kháng thể đặc hiệu kháng nguyên khi gây miễn dịch bằng các epitope kháng nguyên và khi gây miễn dịch bằng cấu trúc nano mang gen mã hóa kháng nguyên kết hợp cấu trúc giầu CpG; (3) Đề tài đã xây dựng được quy trình tạo cấu trúc nano DNA vừa mang cấu trúc giàu CpG vừa mang gen mã hóa kháng nguyên ung thư do đó có thể sử dụng cấu trúc này như một vaccine trong phòng ngừa và điều trị ung thư.

– Các bài báo đã công bố (liệt kê):
1. Duong L.T.T, Phuc P.V., Lua D.T.M, Huyen La Thi, Anh T.T., and Huan Le Quang, (2014). Using the DNA-Chitosan complex as vaccine nanoparticle in cancer. Global Science Research Journal, Vol. 2 (5).pp. 92-97. ISSN: 2408-5502.
2. Anh Dam Tu, Phuc Le Thi, Hien Ngo Thi, Huan Le Quang, (2014). Researching in Immune Response of PolEPCA-2 Recombinant Antigen Specific for Prostate Cancer. Inter. J. Science and Research. (accept. Pub).
3. Ngô T.T. Hiền, Đàm Tú Anh, Lê T.M. Phúc, Lê Quang Huấn, Phạm Thiện Ngọc, ((2014). Nghiên cứu biểu hiện gen mã hóa polyEpitop của kháng nguyên ung thư tiền liệt tuyến sớm (EPCA-2) trên vi khuẩn E. coli. Y học lâm sàng, số 80, trang 74-81.
– Các bằng sáng chế, giải pháp hữu ích (liệt kê):
Tên giải pháp hữu ích: Phức hệ nano có tác dụng vận chuyển thuốc hướng đích và tăng cường đáp ứng miễn dịch trong điều trị ung thư. Ngày nộp đơn: 3/5/2013, Ngày được chấp nhận đơn hợp lệ:16/8/2013
– Các sản phẩm cụ thể (mô tả sản phẩm, nơi lưu giữ): (1) Gen mã hóa các epitope EPCA-2.22 và EPCA-2.19 của kháng nguyên EPCA-2; (2) Gen mã hóa vùng kháng nguyên chứa các epitope của kháng nguyên HER2 và cấu trúc giầu CpG có khả năng tăng cường đáp ứng miễn dịch; (3) Cấu trúc nano chứa các gen mã hóa kháng nguyên ung thư và cấu trúc giầu CpG. Các sản phẩm này đã và đang được lưu giữa tại Phòng Công nghệ tế bào động vật, Viện Công nghệ sinh học, VAST (địa chỉ: phòng 407, nhà B4, 18 Hoàng Quốc Việt, Hà Nội).
– Các sản phẩm khác (nếu có): Đào tạo
1. Th.S. Hoàng Thị Ngà, Đại học Khoa học Tự Nhiên, Đại học Quốc gia Hà Nội. Tên luận văn: “Nghiên cứu chế tạo phức hệ nano-chitosan mang gen mã hóa Telomerase Reverse Transcriptase (hTERT). Đã được cấp bằng ngày 26/4/2014 với số bằng DM 017386.
2. BS. Nội Trú, Ngô Thị Thu Hiền, Đại Học Y Hà Nội. Tên luận văn: “Nghiên cứu biểu hiện gen mã hóa polyepitope kháng nguyên ung thư tiền liệt tuyến sớm (EPCA-2) trên vi khuẩn E. coli. Đã bảo vệ và đã được cấp bằng với số bằng 001485, ngày cấp 6/3/2014
3. NCS. Đàm Thị Tú Anh, Đại Học Y Hà Nội. Tên đề tài: Nghiên cứu tạo kháng thể đặc hiệu kháng nguyên ung thư tiền liệt tuyến ứng dụng trong chẩn đoán”. Đã bảo vệ cấp cơ sở ngày 7/1/2015.

6lequanghuan

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Cấu trúc của đoạn giầu CpG

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Cấu trúc bậc 2 của phân tử DNA tái tổ hợp

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